Our patented technology, JAA-F11 specifically targets the alpha-linked form of a well-known disaccharide tumor marker, the Thomsen-Friedenreich glycoantigen (TF-Ag), found on the surface of 85% of all carcinomas.
Thus, over half of all cancers are expected to be TF-Ag positive, creating a potentially huge market.
TF-Ag is involved in spread of cancer cells. The mechanism of the involvement of TF-Ag in cancer metastasis is shown below. We believe that our highly specific JAA-F11 antibody which reacts with TF-Ag will create a survival advantage for patients with TF-Ag expressing tumors through direct killing of cancer cells and by blocking tumor cell spread. It also will be useful in many cancers to detect metastasis. Studies of 39 human breast cancer cell lines shows reactivity of JAA-F11 with ~85%, including the difficult to treat triple negative breast cancers.
Immunohistochemical analysis shows specific binding to ~85% of the patients' tumors from patients with several types of carcinomas including breast, lung, prostate, bladder, and ovarian.
Clinical translational research of humanized JAA-F11 for antibody-drug conjugate therapy and diagnosis is currently underway. We used the crystal structure of JAA-F11 and carbohydrate threading to create humanized antibody constructs with the same or improved specificity.
The humanized construct selectively kills cancer cells by Antibody Directed Cellular Cytotoxicity, and can internalize rapidly to be used in antibody-drug conjugates. See references below for more detail.
Our technology, the JAA-F11 antibody is targeted immunotherapy addressing the critical need for safe and effective treatment for primary and metastatic Breast Cancer, including the unmet need for treatment of triple negative Breast Cancer.
Our strategic plan is to bring humanized JAA-F11 to first-in human Breast Cancer trials and seek strategic pharmaceutical partnerships with the intent to license our technology.
hJAA-F11 Ab internalizes (white dots) in cancer cells showing capability use in Ab drug conjugate delivery. Credit: Loukia Karacosta
Preclinical Analysis of JAA-F11, a Specific Anti–Thomsen-Friedenreich Antibody via Immunohistochemistry and In Vivo
http://www.transonc.com/article/S1936-5233(17)30300-5/fulltext
Rittenhouse-Olson, K. Future Perspective article. JAA-F11: Extending the life of mice with breast cancer. In p.923-927. Expert Opinions in Biological Therapy 7 (7) 2007.
Olga V. Glinskii, Virginia H. Huxley, Gennadi V. Glinsky, Kenneth J. Pienta, Avraham Raz, and Vladislav V. Glinsky: Mechanical entrapment is insufficient and intercellular adhesion is essential for metastatic cell arrest in distant organs. Neoplasia 7, 522-527 (2005). doi:10.1593/neo.04646
Humanization of JAA-F11, a Highly Specific Anti-Thomsen-Friedenreich Pancarcinoma Antibody and In vitro Efficacy Analysis
https://www.ncbi.nlm.nih.gov/m/pubmed/28830009/?i=2&from=/29477636/related https://authors.elsevier.com/sd/article/S1476558617302270
Adel Almogren, Julia Abdullah, Kshipra Ghapure, Kimiko Ferguson, Vladislav Glinskii, Kate Rittenhouse-Olson (2012). Anti-Thomsen-Friedenreich-Ag (anti-TF-Ag) potential for cancer therapy. Frontiers in Bioscience.S4, 840-863. http://www.bioscience.org/home/eoa/304.pdf
Matthew B. Tessier, Oliver C. Grant, Jamie Heimburg-Molinaro,Snehal Jadey, Andrew Gulick, Kate Rittenhouse-Olson, and Robert J. Woods. Computational Screening of the Human TF-Glycome Provides a Structural Definition for the Specificity of Anti-Tumor Antibody JAA-F11
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